Abeer Ahmed - November 23, 2011

Identification of Novel Protein Targets Regulated by Maternal Dietary Fatty Acid Composition in Mice Liver

We investigated the roles of different n-6/n-3 fatty acid ratios in maternal diet during gestation and lactation periods on the regulation of offspring metabolism. Target identification with proteomics was performed by comparing the protein expression levels in the offspring obtained from mothers fed a 5:1 and 30:1 n-6/n-3 fatty acid ratios. 2D PAGE was used to separate the proteins, which were subsequently identified and fully characterized with MALDI-MS/MS. Prominent differences were observed between the two different diets.

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